Dysfunctionality of a tobacco mosaic virus movement protein mutant mimicking
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منابع مشابه
Dysfunctionality of a tobacco mosaic virus movement protein mutant mimicking threonine 104 phosphorylation.
Replication of tobacco mosaic virus (TMV) is connected with endoplasmic reticulum (ER)-associated membranes at early stages of infection. This study reports that TMV movement protein (MP)-specific protein kinases (PKs) associated with the ER of tobacco were capable of phosphorylating Thr(104) in TMV MP. The MP-specific PKs with apparent molecular masses of about 45-50 kDa and 38 kDa were reveal...
متن کاملStudy on Genetic Diversity of Terminal Fragment Sequence of Isolated Persian Tobacco Mosaic Virus
Tobacco mosaic virus (TMV) is one of the devastating plant viruses in the world that infects more than 200 plant species. Movement protein plays a supportive role in the movement of other plant viruses, and viral coat protein is highly expressed in infected plants and affects replication and movements of TMV. In order to investigate genetic variation in the terminal fragment sequence in Iranian...
متن کاملMimicking carboxyterminal phosphorylation differentially effects subcellular distribution and cell-to-cell movement of Tobacco mosaic virus movement protein.
Phosphorylation of Tobacco mosaic virus movement protein (TMV-MP) at three carboxyterminal Ser/Thr sites negatively regulates TMV-MP gating function and viral spread in Nicotiana tabacum but not in Nicotiana benthamiana, indicating a host dependant inactivation strategy. Here, we examine the effect of mimicking carboxyterminal phosphorylation on cell-to-cell transport of TMV-MP protein itself i...
متن کاملThe tobacco mosaic virus 126-kilodalton protein, a constituent of the virus replication complex, alone or within the complex aligns with and traffics along microfilaments.
Virus-induced cytoplasmic inclusion bodies (referred to as virus replication complexes [VRCs]) consisting of virus and host components are observed in plant cells infected with tobacco mosaic virus, but the components that modulate their form and function are not fully understood. Here, we show that the tobacco mosaic virus 126-kD protein fused with green fluorescent protein formed cytoplasmic ...
متن کاملTransgenic plants expressing potato virus X ORF2 protein (p24) are resistant to tobacco mosaic virus and Ob tobamoviruses.
The p24 protein, one of the three proteins implicated in local movement of potato virus X (PVX), was expressed in transgenic tobacco plants (Nicotiana tabacum Xanthi D8 NN). Plants with the highest level of p24 accumulation exhibited a stunted and slightly chlorotic phenotype. These transgenic plants facilitate the cell-to-cell movement of a mutant of PVX that contained a frameshift mutation in...
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